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KMID : 0620920170490100002
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Experimental & Molecular Medicine
2017 Volume.49 No. 10 p.2 ~ p.2
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Ctbp2-mediated β-catenin regulation is required for exit from pluripotency
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Kim Tae-Wan
Kwak So-Jung
Shin Ji-Hoon
Kang Byung-Hee
Lee Sang-Eun
Suh Min-Young
Kim Jae-Hwan
Hwang In-Young
Lee Jong-Hyuk
Choi Jin-Mi
Cho Eun-Jung
Youn Hong-Duk
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Abstract
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The canonical Wnt pathway is critical for embryonic stem cell (ESC) pluripotency and aberrant control of β-catenin leads to failure of exit from pluripotency and lineage commitments. Hence, maintaining the appropriate level of β-catenin is important for the decision to commit to the appropriate lineage. However, how β-catenin links to core transcription factors in ESCs remains elusive. C-terminal-binding protein (CtBP) in Drosophila is essential for Wnt-mediated target gene expression. In addition, Ctbp acts as an antagonist of β-catenin/TCF activation in mammals. Recently, Ctbp2, a core Oct4-binding protein in ESCs, has been reported to play a key role in ESC pluripotency. However, the significance of the connection between Ctbp2 and β-catenin with regard to ESC pluripotency remains elusive. Here, we demonstrate that C-terminal-binding protein 2 (Ctbp2) associates with major components of the β-catenin destruction complex and limits the accessibility of β-catenin to core transcription factors in undifferentiated ESCs. Ctbp2 knockdown leads to stabilization of β-catenin, which then interacts with core pluripotency-maintaining factors that are occupied by Ctbp2, leading to incomplete exit from pluripotency. These findings suggest a suppressive function for Ctbp2 in reducing the protein level of β-catenin, along with priming its position on core pluripotency genes to hinder β-catenin deposition, which is central to commitment to the appropriate lineage.
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KEYWORD
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Embryonic stem cells, Gene silencing
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